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Numerous studies have reported that a healthy lifestyle can enhance an individual’s lifespan and reduce lifestyle-related diseases. Epigenetic clocks, which measure biological age and are influenced by lifestyle and genes, accurately predict age and have been associated with biomarkers and mortality risk.Recently, researchers have developed DNAmFitAge based on genes related to physical fitness, in which physically fit individuals appear to have a younger biological age and better age-related outcomes. The microbiome, which is a highly adaptable system, changes with age, thereby impacting immunity, inflammation, and longevity.